By R.H. BURDON and P.H. van KNIPPENBERG (Eds.)
This quantity is a finished choice of equipment for the isolation, characterization, research and estimation of soluble lipoproteins. It surveys every one point of lipoprotein know-how in a severe demeanour for you to allow the investigator to choose the tools so much suitable to his requisites. Having made his selection, he'll then discover a specific description of ways to accomplish the procedure.
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Additional info for A Guidebook to Lipoprotein Technique
Abbreviations are as follows: d, the density of the NaCl solution (g/ml). C,, the concentration of salt in mg/ml of solution. C,, the concentration of water in mg/ml of solution. nm, the refractive index of the salt solution for Na, line, at 20 "C. nX. the refractive index for Na, line at 26 "C. qm, viscosity at 20 "C in centipoises. qX, viscosity at 26 "C in centipoises. Mol. 44 Note that the values quoted in this table may differ slightly from those given by Lindgren (1975). This is due to the different method of interpolation that has been used in the preparation of the table.
This time can be considerably reduced by running at 50000 rev/min, when the field at the top of the tube amounts to 149 000 g. 2Ti or 60Ti can only be used in centrifuges of the appropriate rating. If the machines available are not rated to handle these high energy rotors, it may be necessary to use others such as the Beckman 30 or 40 series. The Beckman 35 rotor (total volume 564 ml) can be used to prepare VLDL or LDL, but is inadequate for the isolation of HDL. For this purpose, the 40 rotor (total volume 162 46 A GUIDEBOOK TO LIPOPROTEIN TECHNIQUE ml) can be used.
However, the alternative is not without problems of its own that arise from the fact that many apo-lipoproteins are now known to be heterogeneous. For example, apo-lipoprotein A was eventually found to consist of two separate and quite different proteins that were designated A-I and A-11. At first sight, this would appear to present no difficulty since each can be regarded as an apo-lipoprotein. e. that it can be the sole protein present in a class of lipoproteins. At first, it was thought that proteins such as A-I and A-I1 did not fit this definition and that they required an alternative name, for which the clumsy term constitutive polypeptides was coined.
A Guidebook to Lipoprotein Technique by R.H. BURDON and P.H. van KNIPPENBERG (Eds.)